前言目的选育Vero细胞森林脑炎疫苗适应毒种。方法将森林脑炎疫苗鼠脑病毒“森张”株在Vero细胞上连续传代适应,并对不同代次收获的病毒液的病毒滴度、免疫原性、稳定性等进行检测。结果“森张”株病毒在Vero细胞上传代适应后,病毒滴度达8・5LgLD50/ml;免疫血清中和抗体效价高于1∶20,其它检测项目均符合疫苗生产用毒种的要求。结论Vero细胞适应的“森张”株毒种的初步特性显示可作为制备Vero细胞森林脑炎疫苗用毒种。
ObjectiveToscreenanappropriatevirusseedforthepreparationofVerocell-derivedtick-borneencephalitisvaccine.MethodsThecurrentvirusstrainSen-Zhangforpreparationoftick-borneencephalitisvaccinewassubculturedinVerocells,andthetiters,immunogenicitiesandstabilitiesofvirusofvariouspassageswerestudied.ResultsThevirustiterofSen-ZhangstrainadaptedinVerocellsreached8.5LgLD_50/ml.Theserumneutralizingantibodytiterofmiceimmunizedwiththestrainreachedmoretha...
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