HTB

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WERI-Rb-1（人视网膜母细胞瘤）人视网膜是由位于眼睛后部多层结构组成的。它包括视细胞和视网膜色素上皮细胞（RPE），视网膜色素细胞通过提供营养和代谢废物来滋养视网膜。RPE位于神经视网膜和脉络膜之间，成为血视网膜屏障的外层控制着视网膜下间隙的化学成分，人们发现RPE在许多蛋白质的极性方面不同于其他上皮细胞。几乎所有的上皮细胞的顶生面都没有基质，而RPE的顶生面胞膜是与细胞外基质相接触的。在早期胚胎发育过程中，RPE自发转分化为神经视网膜组织，RPE在其邻近的光感受器的发育和维持中有着重要的作用，光照条件下RPE能产生大量的活性氧，RPE的增殖也是许多眼病发病过程中重要的一步，如增殖性视网膜病变。人视网膜色素上皮细胞（HRPEpiC）提取于人视网膜组织，于第一代冻存。每管含有细胞数>5×105cells/ml，此细胞通过Cytokeratin-18,Cytokeratin-19ａndFibronectin免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。CellBiologyATCC®Number:HTB-169™Price:$255.00Designations:WERI-Rb-1Depositors:TWSeryBiosafetyLevel:1Shipped:frozenMedium&Serum:SeePropagationGrowthProperties:suspensionOrganism:Homosapiens（human）Morphology:grape-likeclustersofroundcellsSource:Organ:eyeTissue:retinaDisease:retinoblastomaPermits/Forms:InadditiontotheMTAmentionedabove,otherATCCand/orregulatorypermitsmayberequiredforthetransferofthisATCCmaterial.AnyonepurchasingATCCmaterialisultimatelyresponsibleforobtainingthepermits.Pleaseclickhereforinformationregardingthespecificrequirementsforshipmenttoyourlocation.RelatedCellCultureProductsIsolation:Isolationdate:1974Applications:transfectionhost（technologyfromamaxatechnologyfromamaxa）Tumorigenic:Yes,inrabbitsCytogeneticAnalysis:Thisisaneardiploidline.Themodalchromosomenumberis47occurringat38%,ａndtherateofpolyploidyis9%.Fifteentosixteenmarkerchromosomesarepresentinallcells.Theyaret（1,?）,t（3p,5q）,der（3）t（?q29;?）,t（3q,?）,5q+,i（6p）,t（7q,?）,9q+,t（10q,21q）,16q+ａndfivetosixothers.Normalchromosomes3,10,13ａnd16areabsent.TherearetwocopiesoftheXchromosome.NoYchromosomesweredetectedinQMstainedpreparationsIsoenzymes:ES-D,1;G6PD,B;GLO-I,2;Me-2,1;PGM1,1;PGM3,0Age:1yearGender:femaleEthnicity:CaucasianComments:TheWERI-Rb-1lineisoneoftwohumanretinoblastomacelllinesestablishedin1974byR.M.McFallａndT.W.Sery.ThecellssurviveculturinginDifcoBacto-Agarbutdonotformcolonies.Scanningelectronmicroscopyrevealssomevariationinthenumberａndfrequencyofsurfaceblebs,lamellipodiaａndmicrovilli.Thelineisofinterestinstudiesofcelldifferentiation,animalmodelsoftumortherapyａndbiochemicalevaluations.Propagation:ATCCcompletegrowthmedium:ThebasemediumforthiscelllineisATCC-formulatedRPMI-1640Medium,CatalogNo.30-2001.Tomakethecompletegrowthmedium,addthefollowingcomponentstothebasemedium:fetalbovineserumtoafinalconcentrationof10%.Temperature:37.0CAtmosphere:5%CO2inairrecommendedSubculturing:Protocol:Culturescanbemaintainedbyadditionoffreshmediumorreplacementofmedium.Alternatively,culturescanbeestablishedbycentrifugationwithsubsequentresuspensioninfreshmediumat2-3X10exp5viablecells/ml.Maintaincelldensitybetween1X10exp5ａnd1to2X10exp6viablecells/ml.

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Mediumrenewal:Every3to4daysPreservation:Freezemedium:Completegrowthmedium,95%;DMSO,5%Storagetemperature:liquidnitrogenvaporphaseRelatedProducts:Recommendedmedium（withouttheadditionalsupplementsorserumdescribedunderATCCMedium）:ATCC30-2001recommendedserum:ATCC30-2020References:23026:McFallRC,etal.Characterizationofanewcontinuouscelllinederivedfromahumanretinoblastoma.CancerRes.37:1003-1010,1977.PubMed:84403623030:McFallRC,etal.Scanningelectronmicroscopicobservationoftworetinoblastomacelllines.CancerRes.38:2827-2835,1978.PubMed:67919032287:RostomilyRC,etal.Expressionofneurogenicbasichelix-loop-helixgenesinprimitiveneuroectodermaltumors.CancerRes.57:3526-3531,1997.PubMed:9270024